General features
| Gene ID | MYPE8250
|
| Type | CDS
|
| From | 1105471
|
| To | 1104359
|
| Strand | -
|
| General name | trmD
|
| Definition | tRNA-(5-methylaminomethyl-2-thiouridylate) methyltransferase
|
| nt length | 1113
|
| aa length | 370
|
| m.w. | 42410.03
|
| COG | [J] Translation, ribosomal structure and biogenesis
|
Orthologs (if any)
| Bacteria* | Reciprocally best hit to | Score | E-value
|
| mpne | MPN422 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase 509863:508751 | 322 | 3.5e-110
|
| mgen | WP_162465265.1 tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma genitalium] | 294 | 1.9e-99
|
| mpul | WP_010925359.1 tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma pulmonis] | 310 | 1.4e-105
|
| mmob | WP_156768871.1 tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma mobile] | 299 | 2.9e-101
|
| mmyc | NP_975433.1 tRNA-specific 2-thiouridylase MnmA [Mycoplasma mycoides subsp. mycoides SC str. PG1] | 349 | 9.4e-121
|
| mgal | WP_011113349.1 tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma gallisepticum] | 306 | 2.4e-100
|
| uure | WP_004026155.1 tRNA 2-thiouridine(34) synthase MnmA [Ureaplasma urealyticum] | 362 | 7.1e-126
|
| phyt | WP_011160500.1 tRNA 2-thiouridine(34) synthase MnmA [Onion yellows phytoplasma] | 269 | 1.5e-89
|
| bsub | NP_390627.3 tRNA-specific 2-thiouridylase [Bacillus subtilis subsp. subtilis str. 168] | 280 | 5.8e-93
|
| cace | WP_010965531.1 MULTISPECIES: tRNA 2-thiouridine(34) synthase MnmA [Clostridium] | 204 | 7.8e-64
|
| ecoli | NP_415651.4 tRNA-specific 2-thiouridylase [Escherichia coli str. K-12 substr. MG1655] | 307 | 8.9e-104
|
*mpne, Mycoplasma pneumoniae; mgen, Mycoplasma genitalium; mpul, Mycoplasma pulmonis; mmob, Mycoplasma mobile; mmyc, Mycoplasma mycoides; mgal, Mycoplasma gallisepticum; uure, Ureaplasma urealyticum; phyt, ; bsub, Bacillus subtilis; cace, Clostridium acetobutylicum; ecoli, Escherichia coli K-12;
Pfam search result:
| Model | No. | Descrip
tion | Position (aa) | Score | E-value
|
| tRNA_Me_trans | 1 | tRNA methyl transferase | 2-364 | 428.6 | 1.5e-128
|
| NAD_synthase | 1 | NAD synthase | 2-91 | 32.9 | 3.5e-08
|
| ThiI | 1 | Thiamine biosynthesis protein (ThiI) | 3-47 | 27.9 | 1.5e-06
|
BLAST search result (top 10 hits)

| ID Description | Identity | Score | E-value
|
| ref|WP_011077646.1| tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma penetrans] | 370/370 (100%) | 1957 | 0.0e+00
|
| ref|WP_004024877.1| tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma iowae] | 199/363 (54%) | 1027 | 1.9e-135
|
| ref|WP_038102516.1| tRNA 2-thiouridine(34) synthase MnmA [Ureaplasma diversum] | 193/365 (52%) | 961 | 2.5e-125
|
| ref|WP_070406665.1| MULTISPECIES: tRNA 2-thiouridine(34) synthase MnmA [unclassified Spiro... | 195/366 (53%) | 934 | 3.3e-121
|
| ref|WP_127093155.1| tRNA 2-thiouridine(34) synthase MnmA [Spiroplasma poulsonii] | 196/366 (53%) | 932 | 6.1e-121
|
| ref|WP_004026155.1| tRNA 2-thiouridine(34) synthase MnmA [Ureaplasma urealyticum] | 188/363 (51%) | 930 | 1.7e-120
|
| ref|WP_162649593.1| tRNA 2-thiouridine(34) synthase MnmA [Spiroplasma sp. TIUS-1] | 196/364 (53%) | 928 | 2.2e-120
|
| ref|WP_040093328.1| tRNA 2-thiouridine(34) synthase MnmA [Spiroplasma poulsonii] | 195/366 (53%) | 928 | 2.4e-120
|
| ref|WP_129620067.1| tRNA 2-thiouridine(34) synthase MnmA [Mycoplasma gallinaceum] | 188/364 (51%) | 923 | 1.3e-119
|
| ref|WP_126821235.1| tRNA 2-thiouridine(34) synthase MnmA [Spiroplasma endosymbiont of Mega... | 193/366 (52%) | 922 | 2.2e-119
|
Maintained by Jun Ishikawa